The learning centre opens at Tesco's Fenny Lock distribution centre in Milton Keynes this month. It will be available to staff from Tesco sites in the area along with their families and friends.
Its inception follows a trial at Tesco's Welham Green distribution centre in conjunction with shopworkers union Usdaw.
Staff will have access to 10 training PCs for a range of courses to help them brush-up on reading, writing and maths skills, as well as online courses supported by UFI Learndirect, the government-sponsored flagship for e-learning.
The instore training computers will give staff who may not have access to computers the opportunity to learn packages such as Microsoft Word, Excel and e-mail applications.
Tesco HR director Clare Chapman said: "We see it as our responsibility to help our staff brush up on life skills such as reading and writing, as well as introduce them to new opportunities."