Instore marketing at Somerfield, Safeway and the Scottish Co-op will advertise an advice helpline, and a website set up to combat the nation's poor record on lifestyle-related health conditions.
The healthy eating campaign, which uses an apple as its logo, has been allocated £1.75m over the next three years by the Scottish Executive. Scotland's food and health co-ordinator Gillian Kynoch said: "We want to get our brand for healthy living, the apple, very well known in Scotland, with TV, press and outdoor advertising starting this week, and instore imagery with the support of retailers."
Sainsbury will tie in with the venture when it relaunches its Taste of Success schools initiative in Scotland at the end of January.
It will promote the campaign's healthy eating messages in the classroom activities which the programme involves.
Kynoch said Scottish food manufacturers were also being encouraged to take fat out of the more traditional lines.
The Food and Drink Federation said it looked forward to working with the Scottish Executive on the campaign.