The retailer moved to a store half the size of its original, in Didcot, last week but insisted the move was because of issues with the lease rather than the size of the store.
"Our existing Didcot store was acquired on a short-term lease. It was great value for us at the time, but our landlords have now secured a long-term tenant and have offered us the opportunity to relocate to the new unit on a 10-year lease," explained MD Andy Latham.
"This move fits well with the learnings we have taken from our first seven months of trading across the business, and we are pleased to be able to continue to offer the same great product ranges that our customers visit us for every day. There are no plans to make any colleagues redundant as a result of the move."
Alworths, which now has 12 stores, targets sites between 3,000 sq ft and 7,000 sq ft. The Didcot store was around 10,000 sq ft.
The relocation comes as the retailer embarks on a wave of store openings. Its 12th store opened this week, in Tiverton, and it plans to open a further two, in Swadlincote and Newhaven, in September.
It aims to have 22 stores by the end of 2010 across the UK, after initially targeting former Woolworths stores in the south east of England.