Its latest animated release, The Wild, is being promoted with on-pack offers in Somerfield. Customers get the chance to win a family holiday for four to Kenya, free zoo tickets and holiday discounts.
The offer appears on packs of grapes, apples, bananas, sweetcorn, beans, sugar snaps, mushrooms, tomatoes, avocados, prepared fruit, strawberries, raspberries, blueberries and citrus fruits, and will run until the end of this month.
Tesco has also begun selling satsumas and clementines that carry collectible stickers of Winnie the Pooh characters, currently on promotion at two for £2
instead of £1.39 each. Two years ago it offered children's packs of 'fun-size' fruit with Disney stickers.
Somerfield produce category buyer Margaret James said: "The promotion was the perfect way to reinforce the 5-a-day message."
A Tesco spokeswoman said: "This new and innovative joint venture with Disney and our supplier is a great way to harness 'pester power' and use it to get kids eating more healthily.
"Because of the great response we have had we'll be looking to roll it out on other fruit soon."
Disney's move towards licensing healthy products is believed to be linked to publicity about childhood obesity. Its ten-year contract with McDonald's runs out at the end of this year.
In the US it has launched Disney Garden - a fresh produce brand for fruit and vegetables linked to Disney characters such as those from The Jungle Book. It will launch several stone fruit lines this month in the US in partnership with Imagination Farms.
Disney licences baby tomatoes at French retailer Champion and mini bananas at Metro in Germany.