The tags can cut the time taken to read a pallet-load by 80%, speeding short shelf life lines through the supply chain.
M&S food business unit director Justin King said: "We will see how this works on produce and roll it out to other categories after Christmas."
The multi-million M&S project runs alongside a general upgrade of supply chain and delivery equipment, geared towards reducing costs.
As Marks and Spencer reported growth this week for a fourth successive quarter, chief executive Roger Holmes said that driving buying efficiencies was a key priority.
Marks and Spencer plans to cut long lead times and streamline distribution depots.